This Paper Aims to Optimize the Bio-Diesel Engine Parameters of Kirloskar Tv1. In India With Increase In Use of Technology, Automobile Sector Is Growing Rapidly and Search For Capable Alternative Fuel Is Required. Biodiesel Is an Alternative to Conventional Diesel Fuel Made from Renewable Resources, Such As Non-Edible Vegetable Oils. No Engine Modifications Are Required to Use Biodiesel In Place of Petroleum-Based Diesel. Petroleum Prices Approaching Record Highs and They Will Deplete Within Few Decades, It Is Clear That More Can Be Done to Utilize Domestic Non-Edible Oils While Enhancing Our Energy Security. Optimization of Bio-Diesel Engine Parameter I.E. Mechanical Efficiency Is Done Using Orthogonal Array of L-9 In Taguchi Analysis. Optimum Levels of Variance from Test Results Are Taken and Confirmed Practically.