Comparative Study on the Viability and Germination of Seeds of Some Selected Medicinal Plants
Exploring the Correlation Between Seed Germination Strategies and Plant Functional Traits
by Manisha .*, Dr. Deepak Kumar,
- Published in Journal of Advances and Scholarly Researches in Allied Education, E-ISSN: 2230-7540
Volume 13, Issue No. 1, Apr 2017, Pages 1118 - 1123 (6)
Published by: Ignited Minds Journals
ABSTRACT
Seed germination strategies vary dramatically among species but relatively little is known about how germination traits correlate with other elements of plant strategy systems. Understanding drivers of germination strategy is critical to our understanding of the evolutionary biology of plant reproduction.We present a novel assessment of seed germination strategies focusing on Australian alpine species as a case study. We describe the distribution of germination strategies and ask whether these are correlated with, or form an independent axis to, other plant functional traits. Our approach to describing germination strategy mimicked realistic temperatures that seeds experience in situ following dispersal. Strategies were subsequently assigned using an objective clustering approach. We hypothesized that two main strategies would emerge, involving dormant or non-dormant seeds, and that while these strategies would be correlated with seed traits (e.g., mass or endospermy) they would be largely independent of vegetative traits when analysed in a phylogenetically structured manner.
KEYWORD
seed germination strategies, viability, germination, medicinal plants, plant strategy systems
INTRODUCTION
Seeds are the crucial part of vegetation‘s without which it can't finish its life cycle. Over the span of plant development seeds rise as a transformative unit that installs inside it another vegetation A seed's achievement lies in foundation of another plant. In any case, some of the time this doesn't occur and seeds neglect to develop over an extensive stretch of time. The disappointment of seeds to grow under horrible natural conditions speaks to the calm condition of a seed. In actuality, when seeds neglect to grow under ideal ecological conditions, it is called torpidity. Lethargic seeds don't develop in a predefined timeframe when exposed to typical physical ecological variables viz., temperature, light/dim and so on, which are generally ideal for their germination The physiological phase of each seed in a great deal changes from each other. This methodology encourages them to stay away from rivalry among them and furthermore give a preferred position in defeating unconducive conditions, so all people don't get wiped out Seed torpidity, notwithstanding, is an attractive trademark that help in dispersal of seeds over more noteworthy land separations during their lethargy period without losing their suitability. It, anyway becomes unfortunate when the degree of torpidity is extremely huge thusly meddling with the opportune foundation of a plant. Nearness of torpidity in a seed isn't just connected with the nonattendance of germination yet in addition with the conditions required for germination During germination, a piece of the undeveloped organism known as radical, which is the future root, broadens first and infiltrate its encompassing tissues for example the seed coat. The incipient organism and the endosperm are the two significant segments of a seed The communication of these significant parts helps in seed germination and further foundation of the seedling in a domain. Seeds utilize various instruments to comprehend the natural sign, which learn whether the conditions are ideal for germination or not A total seed is a blend of maternal and fatherly genome shaping a mind boggling structure.
SEED VIABILITY
Seed practicality is the capacity of the undeveloped organism to grow, and is influenced by various conditions. An assortment of elements can influence seed practicality, for example, the capacity of the plant to deliver reasonable seeds, predator and pathogen harm, and natural
organisms and, after some time, cells kick the bucket and can't be supplanted. The measure of time a seed stays feasible can be impacted by both hereditary qualities and condition.
EXPLORATION AND COLLECTION OF PGR OF MEDICINAL AND AROMATIC PLANTS
During the period 1975-1996, over I ,SOO germplasm assortments of medicinal and fragrant plants had been gathered through plant investigations in a portion of the conspicuous medicinal and sweet-smelling plants by the NBPGR, to be specific, Isabgol (Plantago ovata}- - 80 assortments from Gujarat; opium poppy (Papavarsomniferum}- - 140 assortments from Gujarat, Madhya Pradesh and Uttar Pradesh; Palmarosa (Cymbopogon martinii}- - 62 assortments from Maharashtra and Madhya Pradesh; Vetiver (Vetiveriazizanioides }- - 11S assortments from Rajasthan and Utter Pradesh. Moreover, over SSO germplasm promotions from Uttaranchal slopes, and from Kerala and more than 100 increases from Kanger valley, 'Keshkal', Kanker hold woodland territories in Bastar (Chattisgarh) were additionally made. notwithstanding the over, 264 germplasm promotions of M&AP were additionally gathered from Katarmiaghat, Nishangarh, Lakhimpur, Rishikeshi Haridwar, in Uttar Pradesh, Uttaranchal and Chattisgarh (save backwoods in Bastar) under G-IS communitarian venture. The subtleties have just been featured (Gautam et ai, 2000,20 Glorious Years of NBPGR 1976-1996). These assortments have been developed, kept up! assessed for different financial characters.
PLANTS AS A SOURCE OF MEDICINES
Nature has given a rich storage facility of home grown solutions for fix all humanity's evil. All through the world individuals have used a few a great many plants and plant items as fix 11 for human afflictions. In the plant realm, practically all plants are medicinal and the use of medicinal plants particularly in customary medication is very much perceived (Chaudhary and Tariq, 2006). In the creating nations, drugs are costly, yet additionally have many reactions during treatment for any scatters that is the reason in the present period it is being underlined to look through medicinally important plants. India has one most seasoned, most extravagant and assorted social customs related with the utilization of plants and herbs for human, animals and plant wellbeing. A significant number of the elements of Indian cooking which have been passed on from ages contain medicinal properties (Chakraborty and Das, 2010). In India, the 'Ayurvedic System of Medicine' has been medicinal plants. 'Charaka Samhita' and 'Susruta Samhita' which are their restorative works are one of the regarded fortunes of writing of indigenous medication today.
MORPHOLOGY OF MEDICINAL PLANTS
Pergulariadaemia (Forsk) Chiov. Family: Asclepiadaceae Common names-Utaran, Gutguti Morphology: The plant is antiquated tomentose and twinning under shrubbery. The stem is terete and much expanded. Leaves are backwards, reniform-hail about 2.5 to 6.5 cm with significantly cordate base with balanced folds and hone apex. The leaves are glabrous and pubescent underneath. The petiole is 2-3 cm long and pubescent. Blossoms are planned in hanging, corymbose cymes ending up being racemes. The peduncles are up to 20 cm long, fuzzy. The pedicels are up to 2-2.5 cm long, filiform and villous. Bracts are lanceolate, extreme, thickly pubescent, 1.5-2 cm long. Sepals are ciliated, acclaim lanceolate, long, serious, around 2-2.5mm long. Corolla is dull greenish yellow or white, glabrous on outside and shaggy inside and around 10-12mm long. Corolla tube is half as long as the elliptic folds. Crown staminal with outer five lobed film and internal on a level plane compacted projections. The pollinia are oval. The natural items are follicles, unequivocally refluxed, lanceolate about 5.8-1-1.2 cm having long nose with fragile spines, pubescent, flotsam and jetsam dim concealing and purplish tinge. Seeds are 6-7 mm long, thickly pubescent, Silky white and 2.5-3cm long.
REVIEW OFLITERATURE
Seed Dormancy in Medicinal plants
Seed torpidity is viewed as an inherent square that confines the culmination of germination of an unblemished practical seed under positive conditions (Finch-Savage and Leubner-Metzger, 2016). A non-torpid seed, in any case, is the one that has the ability to sprout over the amplest scope of typical physical ecological variables (i.e., temperature, light/dim, and so on.) experienced by the genotype (Baskin and Baskin, 2004b). Germination then again is a wonder of bulge of the radicle through the charming cells of the seed to the outside condition. Seed torpidity and germination assumes a urgent job in the foundation of a plant. There are numerous significant medicinal plants that have seed torpidity and furthermore have low germination potential. A great deal of work has been done for improving seed germination and breaking of torpidity since the hour of germination of medicinally significant plant Cistanchedeserticola. In that capacity Mirzaei et al. (2013) contemplated acceptance of seed germination in Robiniapseudoacacia. Copete et al. (2011) examined the physiology, morphology and phenology of seed torpidity break and germination of endemic and uncommon Narcissus hispanicus plant. Scholten et al. (2009) did his work in Lomatiumdissectum which is utilized in various incendiary issue of respiratory framework. Van Klinken et al. (2008) contemplated on seed torpidity discharge system of perpetual leguminous plant Parkinsonia aculeata. Though, Fang et al. (2006) examined the strategies to break seed torpidity in Cyclocaryapaliurus, which is a medicinally significant plant in China. The transient circulation of plants becomes fruitless when lethargy wins (Nonogaki, 2014). The plants that we need in huge number for business purposes should be engendered as and when required. Besides, preservation of plants is vital for satisfying the feasible improvement objectives of United Nations. Preservation of common assets and the ability to use them in supported way are basic for the prosperity and proceeded with endurance for person.
Due to over misuse and living space annihilation various plants are confronting consistent risk of elimination. Practically 80% of the individuals of creating nations depend on conventional medication for essential human services and about 85% of this drug includes the utilization of plant separates. The World Health Organization (WHO) evaluated the expanded interest for medicinal plant-based crude materials at the pace of 15-20% yearly (Sharma and Thokchom, 2014).
TYPES OF DORMANCY
Torpidity is a typical factor in the vast majority of the seeds. The kind of torpidity relies upon seed's interior structures, job of seed covering layers and physiological prerequisites for breaking lethargy, including the impacts of plant development controllers (Baskin and Baskin, 2004a; Nikolaeva, 1969). Physiological torpidity (PD) is the most bottomless kind of seed lethargy found in gymnosperms and significant angiosperm clades. Truth be told, it is the most widely recognized sort 'in field' and 'off field' (i.e., in the research center). Baskin et al. (2015) watched profound physiological torpidity in Leptecophyllatameiameiae while Finch-Savage et al. (2013) recognized it in Acer platanoides. Finch-Savage et al. (2014) additionally watched middle of the road PD in Acer pseudoplatanus. Baskin and Baskin (2015) discovered non-profound PD in Gentianella
physiologically lethargic thus they don't require a lethargy breaking pretreatment for germination. Morphophysiologically torpid (MPD) seeds have immature undeveloped organisms notwithstanding physiological lethargy. Along these lines, they require lethargy breaking pretreatments so as to grow. Baskin and Baskin (2004a) grouped MPD into nine sub types, i.e., (I) non-profound basic MPD; (ii) middle of the road basic MPD; (iii) profound straightforward MPD; (iv) non-profound basic epicotyl MPD; (v) profound basic epicotyl MPD; (vi) profound basic twofold MPD; (vii) non-profound complex MPD; (viii) halfway complex MPD and (ix) profound complex MPD. Non-profound basic MPD was seen in Chaerophyllumtainturieri by Baskin and Baskin (2012). Baskin and Baskin (2011) watched profound basic MPD in Jeffersoniadiphylla. Though, non-profound basic epicotyl MPD was seen in Viburnum odoratissimum and V. nudum and profound straightforward epicotyl MPD was seen in Viburnum acerifolium, V. dentatum and V. dilatatum, (Baskin et al., 2018). Non-profound complex MPD was seen in Symphoricarposorbiculatus by Hidayati et al. (2013) and profound complex MPD was seen in Thaspiumpinnatifidum by Baskin et al. (2014), Fraseracaroliniensis by Threadgill et al. (2013). Another torpidity type is physical lethargy (PY) which is portrayed by seeds having at least one water-impermeable layers of palisade cells (Baskin et al., 2014). This sort of torpidity was found in Acacia fragrance, A. caven by Venier et al. (2012); in Cuscutaaustralis by Jayasuriya et al. (2018) and so on. Combinational torpidity then again is a sort of lethargy where notwithstanding impermeable seed coat the incipient organism is physiologically lethargic (Baskin and Baskin, 2004a; Baskin and Baskin, 2014). Baskin and Baskin (2015) watched this sort of torpidity in Geranium and Trifolium seeds.
Prescription started to isolate from the megico-profound world in created societies by about 500BC. Hippocrates (460-377 BC), referred to all around as the dad of medication, believed disease to be a characteristic as opposed to a heavenly wonder, and henceforth, he felt that prescription can be given without megico-custom way. The most punctual Chinese therapeutic content written in the first century BC was Yellow Emperor's Classic of Internal Medicine. The most established archived record so far on medicinal employments of herbs is the Chinese pharmacopeia, the Pun-Tsao (composed preceding 2500 BC and distributed in 1600 AD by Li Shi Chin). The medicinal plants referenced in the Pun-Tsao incorporate the Indian hemp, aconite, opium, poppy, and croton. The utilization of Ephedra having antihistamine property and Ginseng (Panax pseudo ginseng) can be followed back to old
such customs of employments of herbs are additionally known in Egypt since 1500 BC and in Greece since 300 BC (Kirtikar and Basu, 1918). In India, the Vedas are the epic ballads expounded on 1500 BC. These volumes contain rich material on the home grown legend of that time. Antiquated Indian writing like Rigveda (4000-1500 B.C.), Atharveda (1500B.C.) and Ayurveda (1000-600 B.C.) notice a few medicinal plants and their employments. The Vedas were followed in around 700 BC by the Charaka Samhita, composed by the doctor Charaka, known as father of Indian drug, which is another soonest treatise on Ayurveda, is one of the important Indian records on medicinal plants and their employments. This therapeutic treatise incorporates subtleties of around 350 home grown prescriptions; among them are Visnaga (Ammi visnaga), a herb of Middle Eastern cause that has as of late demonstrated powerful in the treatment of asthma, and Gotu Kola (Centellaasiatica), which has for some time been utilized to treat uncleanliness. The ascent of Buddhism gave a stimulus to the investigation of medication in India. The Buddhist preachers carried with them medications of different countries to India, and in this way enhanced the Materia Medica of Hindu doctors and priests, additionally began the development of medicinal plants in the home grown nurseries of their religious communities during 500 A.D. After 1200 A.D there was a time of decrease in the examination of medicinal plants in India because of intrusions of Mohammedans and the Arabic drug accomplished more conspicuousness during this period (Ainslie, 1813). From 1800 A.D. onwards, the European attack brought about the presentation of western allopathic arrangement of medication in India (Kirtikar and Basu, 1918). Western biomedicine had sway on conventional practices and unquestionably profited in numerous regards. The reasonable absorption of logical standards and strategies into customary home grown mending rehearses offers the possibility of refinement the powerful treatment. Be that as it may, during British principle in India, Western drug was acquainted as a methods with supplant Ayurveda. Prior to 1835, the western doctors and their Indian partners were trading information; from that point, just western medication was perceived as authentic and the Eastern frameworks were effectively disheartened (Mushtaq, 2009). In India, the most old and commended treatise on Hindu Medicine is no uncertainty the Ayurveda, signifying 'the Knowledge of life'. It was composed structure more than 2,000 years prior in India, and was said to be the world prescription. Vedic writing pursued like Charaka (Charak-Samhita, 100-500 AD) and Shushruta (Shushrut-Samhita, 200-500AD) marks the early period of natural science in India. At properties. In Valmiki Ramayana, there is a reference to the presence of uncommon medications like Sanjivani, Savarnyakarni, Sandhankarni and Vishalyakarni in Kanchan slopes and Kailash Mountains. It is a very obvious that Ayurveda, the antiquated study of medication, has its inception in Himachal Pradesh (Chauhan, 2012). One of the old herbs Sarpagandha (Rauvolfia serpentine) still discovers use today, which was utilized in India against lunacy and various types of psychological instability since hundreds of years. So also, the utilization of Chalmogra oil (Hydnocarpuskurzi) to fix uncleanliness is a legend (Ainslie, 1813; Shaughnessy and Wallich, 1844).
RESEARCH METHODOLOGY
The develop products of all the five medicinal plant species as referenced in Table 1 were gathered at various timespans. The products of Rauvolfiaserpentina, R. tetraphylla, Solanum macranthum, Withania somniferous and Caesalpinia bonducella were individually gathered in the long stretch of June, July, November, February and January dependent on the fruiting time of the chose plants (Photo plate 1, 2, 3, 4 and 5).
Table 1: Name of the medicinal plants and their collection site.
ANYLASIS
Seed Viability
Hundred percent seed suitability was not seen in any of the exploratory seeds (Table 2). S. macranthum seeds indicated most elevated feasibility (96.67±0.95%) trailed by W. somnifera (83.33±0.26%), R. tetraphylla (82±0.5%), C. bonducella (70±0.05%) and R. serpentina (67±0.05%) (Photo plate 6-9). The variety in seed practicality among the contemplated seeds was factually noteworthy (P<0.05). The regular seed germination level of the exploratory plants was less when contrasted with the inborn seed suitability of the individual plants. In S. whether the seeds showed 83.33% practicality.
Photo plate 1: Embryo of R. serpentinaplant. a) Viable embryo; b) Non- viableembryo.
CONCLUSION
The preservation and rebuilding of plants is important to keep up an equalization in the biological condition. The vast majority of the plants that are financially or medicinally significant are confronting threat of annihilation because of overexploitation of these plants in nature. Also, a few plants neglect to proliferate normally because of the nearness of basic torpidity inside the seeds. It is a significant obstruction in the rebuilding and preservation process. A definitive job of seeds is to deliver posterity and keep up the species wealth. Seed torpidity doesn't enable the seeds to develop in required time. The medicinal plants those are much of the time required in pharmaceutical enterprises for their important items should be proliferated on enormous scale. The present examination was done to decide the sort of torpidity in the chose medicinal plants to be specific, R. serpentina, R. tetraphylla, S. macranthum, W. somnifera and C. bonducella. These plants are of high medicinal worth. The suitability trial of seeds indicated that seeds of every one of these plants delivered quality seeds for spread. Be that as it may, even now they are not satisfactorily proliferated to fulfill their developing need because of the common lethargy of seeds.
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Corresponding Author Manisha*
Research Scholar surender2485@gmail.com