Antioxidant Activity of Porana Paniculata (Convolvulaceae) – An Ethnomedically Important Plant

Porana paniculata Roxb. (Convolvulaceae) possesses its significant use in the ayurveda among folklore but its phytopharmacological nature has unrevealed (Pullaiah et. al., 1997). Pyrrolizidine alkaloids, and ergoline alkaloids and anthocyanins were also reported (Khare CP, 2007; Rastogi et al. 2007). In the present analysis, after comprehensive literature review, attempts have been made to systematically demonstrate Porana panicula's total flavonoid and total phenol content and antioxidant activity.

• Ethanol • Distilled water • Petri dish • Rota Evaporator • Desicator • Methanol • Aluminium chloride • Potassium acetate • UV / visible spectrophoto meter • Quercetin solution • Folin Ciocalteu reagent • Gallic acid • Aqueous Na2Co3 Methods: For the present study, the entire plants were collected and 1000 gm of its powdered were extracted by cold maceration method with ethanol : water (3:2) mixture as solvent. The maceration proceeded for 72 hours after the contents of the Rota evaporator were filtered and condensed. A resinous greenish extract was obtained and stored in desiccator till further study (Kokate, 1994; Khandelwal, 2005)·

Aluminium chloride colorimetric method was used. 1.5 ml of methanol, 0.1 ml of 10 percent aluminium chloride, 0.l ml of 1 m potassium acetate and 2.8 ml of purified water is combined with the plant extract. The absorption of the reaction mixture was calculated at 415 nm using a double beam UV / visible spectrophotometer and was held at room temperature for 30 minutes. The calibration curve was prepared by planning a concentration of 12.5 to 100 mg/ml of quercetin solution in methanolol (Pourmorad et al. 2006)

It was calculated by the reagent Folin Ciocalteu. The diluted extracts of plants have been mixed with Folin Ciocalteu (5 ml, 1:10 water distilled) and Na2CO3 aqueous reagent (4 ml, 1 M). The mixtures were

In addition to 0.5 ml HAPP and HAIQ solutions at different concentration i.e. 20, 40, 60, 8o and 100 μg/ml, the scavenging potential of the natural radical DPPH antioxidants of the plant extract was calculated to achieve the safe, radical DPPH free radical. The mixture had been shaken and should stay in the dark for 30 minutes at room temperature. The emission in a spectrophotometer was then calculated at 517 nm. L - the normal usage of ascorbic acid. The percentage inhibition was calculated by using following formula - % DPPH Scavenging activity = 100 x AC – AS/AC where, AC = Absorbance of the control, AS = Absorbance of reaction mixture.

In 1000 gm of plant sample there was a broad green viscous matter of approximately 28,9 gm and a proportion of 2,89 percent w per w/w. Total flavonoids of HAPP and HAIQ mg/ml of quercetine were find 59,86±0,54 while total phenols had a total phenol content of 33,34±0,37.

DPPH (diphenyl picryl hydrazine) radical scavenging activity of HAPP, HAIQ and Standard ascorbic acid are presented in Figure -l. In this assay, the antioxidant was able to reduce the stable radical DPPH to yellow 1, 1-diphenyl-1, 2-picryl hydrazine. The molecule of 2, 2-diphenyl -l - picryl hydrazine is characterized as a stable free radical by virtue of the delocalization of the spare electoral over the molecule as a whole. The DPPH free radical proton transfer reaction by a scavenger (A-H) causes absorption to be reduced to 517 nm, accompanied by a typical spectrophotometer collection in the visible region. The effect of antioxidants on DPPH is thought to be due to their hydrogen donating ability.

Pullaiah T. & Moulali D. (1997). Flora of Andhra Pradesh. Hyderabad Scientific publisher. Khare C. P. (2007). Indian Medical Plants Spinger. Rastogi R. P. & Mehrotra, B. N. (2007). Compendium of Medicinal plant, CSIR. Kokate C. K. (1994). Practical pharmacognosy, Vallabh Prakashan, New Delhi. Pourmorad F., Hesseinimehr S.J., Shabebimajd N. (2006). Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. Journ. Biotechnol. 5 (11): pp. 1142-45.