Comparative UV-Spectroscopic and Chemical estimations of Atropine Alkaloid present in Datura stramonium found in Saudi Arabia
DOI:
https://doi.org/10.29070/0vpzhf18Keywords:
Datura stramonium, Saudi Arabian Region, Atropine, Quantitative estimation, UV Spectrophotometric MethodAbstract
Aim: To find out the concentration of Atropine alkaloid in Datura stramonium of Saudi Arabian Region and to develop a new method of the Atropine estimation.
Methods: The presence of Atropine in the alcoholic extract of leaves of Datura stramonium was known by Qualitative TLC profile. Quantitative estimation of Atropine in the extract was done with UV Spectrophotometric Method (new proposed method for determination of Atropine in plant extract) and Titrimetric Method (Pharmacopoeial method) and results were compared.
Results: Qualitative (TLC) Result showing the presence of Atropine in the Datura stramonium extract. After applying the calculations the concentration of Atropine was found to be 0.3% (w/w). Since the both quantitative results were comparable and the values of the results were approximately equal supporting the validation of New Method.
Conclusion: The Saudi Arabian geographical variety of Datura stramonium contains the Atropine Alkaloid as 0.3% (w/w) which is close to the reported value. The new method for the quantitative estimation of Atropine i.e. UV spectroscopic method was developed successfully.
References
Mammen D, Daniel M, Sane RT: Seasonal and Geographical Variations In Chemical Constituents of Leptadenia reticulate, International Journal of Pharmaceutical Sciences Review and Research, 2010 Volume 4, Issue 2
Anonymous: Soils, Plant Growth and Crop production- Medicinal and Aromatic Plants 2010, Volume II (Encyclopedia of Life Support Systems)
Shah Biren, Seth, A.K.: Drug Containing Alkaloids, Textbook of Pharmacognosy and Phytochemistry 2010, First edition, Elsevier Publication
Ali, M.: Alkaloids, Textbook of Pharmacognosy, 2007, CBS Publishers & Distributors
Kirtikar JD, Basu BD. Indian medicinal plants. Allahabad: Lalit Mohan Basu; 1994. pp. 1229–1231.
Research J. Pharmacognosy and Phytochemistry 2013; 5(3): 143-148 O. M. Singh, et al.
Abdollahi M, Karimpour H and Monsef – Esfehani H P, Antinociceptive effects of Teucrium polium L, total extract and essential oil in mouse writhing test, Pharmacology Research.48; 2003: 31- 35
Das S, Kumar P, Basu SP. Review article on phytoconstituents and therapeutic potentials of Daturastramonium linn. J Drug Del Therap. 2012;2(3):4–7.
Pretorius E, Marx J. Datura stramonium in asthma treatment and possible effects on prenatal development. Environ Toxicol Pharm. 2006;21(3):331–337
Taha SA, Mahdi AW. Datura intoxication in Riyadh. Trans R Soc Trop Med Hgy. 1984;78:134–135.
Takhi D, Ouinten M. Study of antimicrobial activity of secondary metabolites extracted from spontaneous plants from the area of Laghouat, Algeria. Adv EnvironmBiol. 2011;5(2):469–476.
Balachandran P, Rajgopal G. Cancer—an ayurve dicperspective. Pharm Res. 2005;51(1):19–30.
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