Study on Molecular Mechanisms of Genome Dynamics in Human Cancer Cells

Characterization and Applications of the HEK293 Cell Line

Abstract

The HEK293 human cell heredity is broadly utilized in cell science and biotechnology. Here we utilize entire genome resequencing of six 293 cell lines to think about the elements of this aneuploid genome in light of the controls used to create normal 293 cell subordinates, for example, change and stable clone age (293T) suspension development adjustment (293S) and cytotoxic lectin choice (293SG). Surprisingly, we see that duplicate number adjustment location could distinguish the genomic area that empowered cell survival under particular conditions (i.c. ricin determination). Besides, we present techniques to distinguish humanvector genome breakpoints and an easy to understand representation apparatus for the 293 genome information. We likewise build up that the genome structure organization is in unfaltering state for a large portion of these phone lines when standard cell refined conditions are utilized. This asset empowers novel and progressively educated investigations with 293 cells, and we will circulate the sequenced cell lines to this impact.