Isolation and Optimization of Lignocellulose Degrading Fungi: Isolation, identification, and optimization of lignocellulose degrading fungi for cellulase production

Abstract

The aim of the present study was to determine the type offungus that can grow and degrade lignocellulose. The research conducted bycarrying out isolation, identification and screening of cellulolytic fungi fromcollected soil samples. Plate screening was done for carboxymethylecellulaseactivity using Congo red dye and zone of cellulose hydrolysis was apparent   as clear area diameter observed. The resultsshowed that among total of 12 isolated fungi maximum CMCase activity was foundin Aspergillus sp. R-2, Penicillium sp. R-2 and Trichoderma sp. R-1 recommendedfurther for cellulase production research.