A Stability-Indicating High Performance Liquid Chromatographic Assay For the Simultaneous Determination of Atenolol and Lercanidipine

Development and validation of a stability-indicating HPLC assay for the simultaneous determination of atenolol and lercanidipine in commercial tablets

Authors

  • Suresh Kumar Kankanala CMJ University, Shillong, Meghalaya Author

Keywords:

stability-indicating, high performance liquid chromatographic assay, atenolol, lercanidipine, reversed phase, HPLC method, commercial tablets, chromatographic separation, phenomenex Gemini C18, acetonitrile, buffer, potassium dihydrogen phosphate, flow rate, UV detection, linearity, proposed method, degradation products, stress studies

Abstract

A simple, rapid, precise and accurate isocraticreversed phase stability indicating HPLC method was developed and validated forthe simultaneous determination of atenolol and Lercanidipine hydrochloride incommercial tablets. The chromatographic separation was achieved on phenomenexGemini C18 (250×4.6 mm, 5 µm) column using a mobile phase consisting ofacetonitrile and buffer (20 mM potassium dihydrogen phosphate pH 3.5) in theratio of (55:45, v/v) at a flow rate of 1.0 ml/min and UV detection at 235 nm.The linearity of the proposed method was investigated in the range of 40-160µg/ml (r2 =0.9995) for atenolol and 8-32 µg/ml (r2=0.9993) for Lercanidipine. Degradation products produced as a result of stressstudies did not interfere with the detection of atenolol and Lercanidipine andthe assay can thus be considered stability-indicating.

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Published

2012-11-01

Issue

Vol. 4 No. 7 (2012): Journal of Advances in Science and Technology (JAST)

Section

Articles