A Analysis on Various Strategies of High Performance Liquid Chromatography

Exploring Separation Strategies in High Performance Liquid Chromatography

Abstract

Chromatography is an analytical technique based on theseparation of molecules due to differences in their structure and/orcomposition. In general, chromatography involves moving a sample through thesystem over a stationary phase. The molecules in the sample will have differentaffinities and interactions with the stationary support, leading to separationof molecules. Sample components that display stronger interactions with the stationaryphase will move more slowly through the column than components with weakerinteractions.