Electrofusion and Regeneration of Protoplast In Potato

Exploiting Electrofusion and Regeneration for Potato Crop Improvement

Abstract

Somatic hybrids obtained directly after fusion containsall organelles from the cytoplasms of both parents. Somatic hybridization viaprotoplast isolation, electrofusion and regeneration is a useful tool totransfer polygenic traits such as late blight resistance in a single step. Itenables a development of tetraploid somatic hybrid between diploid wild speciesand dihaploid of common potato. As a result, tetraploid somatic hybrids may beutilized in conventional breeding for late blight resistance and improvement ofother traits.