A Comparative Analysis Upon Performance of Methicillin Resistant Staphylococcus Aureus: a Case Study of Orthopaedic Patients

Detection and Characterization of Methicillin Resistant Staphylococcus aureus in Orthopaedic Patients

Abstract

Methicillin resistant Staphylococcusaureus (MRSA) is now a threat to both the hospitalized patients andcommunity globally. This work was aimed at detecting molecularly, methicillinresistant Staphylococcus aureus fromthe orthopaedic patients. Conventional biochemical methods were used toidentify the isolates while API STAPH identification test kit furthercharacterized the isolates to species level. The susceptibility test wascarried out using disc agar diffusion method while beta – lactamase productionwas tested for using nitrocefin. Methicillin resistance was detectedphenotypically using cefoxitin 30 μg disc and oxacillin agar screen test.Multiplex polymerase chain reaction (PCR) was used to detect mecA gene, the gene codingmethicillin resistance and blaZ gene,the gene coding for beta- lactamase production with 16SrRNA gene being the internal control.