A Preliminary Study on Oil Biodegradation Using Microbes Isolated From Various Ecological Sites

Evaluation of Oil Biodegradation by Microbes Isolated from Various Ecological Sites

Abstract

In and around Navi Mumbai, hydrocarbon-degrading microorganisms were isolated from ten oil-polluted regions. As a specific enrichment medium with engine oil as a single source of carbon and electricity, Bushnell and Hass broth was used and 19 distinct isolates were collected. Using the Emulsification Index and Gravimetric study, the oil degradation ability of these isolates was evaluated. The GC-MS analysis demonstrated that the conversion of Heneicosane to hexadecane suggests that the organism partly degrades the oil. Biochemical analyses and 16s RNA sequencing verified that the isolate is Bacillus cereus.