Formulation and Evaluation of Herbal Nanoemulsion for Mosquitocidal Activity Against Dengue Vector Aedes aegypti
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The increasing resistance of Aedes aegypti to conventional insecticides has necessitated alternative vector control strategies. This study investigates the formulation and efficacy of a herbal nanoemulsion containing essential oils with mosquitocidal properties. The nanoemulsion was prepared using an ultrasonic emulsification method and characterized for particle size, zeta potential, and stability. Larvicidal and adulticidal bioassays were conducted to evaluate its effectiveness against Aedes aegypti. Results indicated significant mortality rates at low concentrations, demonstrating the potential of herbal nanoemulsions as an eco-friendly and sustainable mosquito control strategy. This study provides insights into the development of natural, nano-based solutions for dengue vector management.
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- Abeyewickreme, W., Wickremasinghe, A.R., Karunatilake, K., Sommererfeld, J. and Axel, K.(2012). Community mobilization and household level waste management for dengue vector control in Gampaha district of Sri Lanka; an intervention study. Pathog. Glob. Health, 106(8); 479 -87.
- Alejo, A.J., Prez, A.M.,Anguilera, E.N. Moreno, M.F. et al., (2017). Pupal productivity in rainy and dry seasons: finding from the impact survey of a randomized controlled trail of dengue prevention in Guerrero, Mexico. BMC Public Health, 17(Suppl 1); 428; 71-78.
- Boornema, A.R. and Murugan, S.T.K.(2018). Breeding habitats of Aedes aegypti mosquitoes and awarwness about prevention of dengue in urban Chidambaram: A cross sectional study. Int. J. Community Med. Pub.Helth, 5(10); 4584-4589.
- Getachew, D., Tekie, H., Michael, T.G., Balkew, M. and Mesfin, A.(2015): Breeding sites of Aedes aegypti: Potential dengue vectors in Dire Dawa, East Ethiopia. Interdisciplinary Perspectives on Infectious Disease. Volume 2015,article Id 706276: 1-8.
- Gomez, Ade, C., deSouza, J.M., Bergamaschi, D.P., Dos Santos, J.L. et al., (2005). Anthropophilic activity of Aedes aegypti and Aedes albopictus in areas under control and surveillance. Rev.Saide Publica., 39(2); 206-10.
- Jomom, K.V., Sudharmini, S. and Valamparampil, T.T,(2009). Aedes mosquitoes in Arboviral epidemic prone area of Kottayam district, Kerala, India. Academic Review, 16; 171-178.
- Joseph, C.C., Ndoile, M.M.,Malima, R.C. and Nkuniya, M. H. M. (2004). Larvicidal and mosquitocidal extracts a coumarin, isoflavonoids and pterocarpans from Neorantanenia mitis., Trans R. Soc. Trop. Med. Hyg., 98,451-455.
- Kabra, S.K., Pandey, A., Broor, S. and Guleria, R.(2007). The evolution of dengue over a decades in Delhi. J. Clin Virol, 40; 87-8.
- Kanhekar, L.J., Ravikumar, R., Meena, R.K., Sharma, R.S., Sharma, A.K. and Enkatesh (2016): Population dyanamics of Aedes aegypti and Aedes albopictus in Jagdalpur city, Baster district of Chattisgarh state, India. Int. J. Mosqt. Res. 3(6):40-44.
- Mariappan, T., Selvam, A., Rajamannar, V. and Arunachalam, N.(2017). Susceptibility of dengue/ Chikungunya vector, Aedes aegypti against carbamate, Organochlorine, Organophosphate and Pyrethroid insecticides. J. Environ. Biol., 38:251-255.
- Mciel de Freitas, R., Marques, W.A., Peres, R.C., Cunha, S.P., De Oliveira, R.L. (2007). Variation in Aedes aegypti (Diptera; Culicidae) container productivity in a slum and a suburban district of Rio de Janeiro during dry and wet seasons. Mem. Inst. Oswaldo Cruz, 102(4); 489-96.
- Meena, A.R. and Choudhury, N.L.(2019). Container breeding preference of Ae. albopictus in Urban environment. Int. J.Mosq. Res., 6(5); 44-47.
- Mehendale, S.M. Risbud, A.R., Rao, J.A., Banerjee, K., (1991). Outbreak of dengue fever in rural areas of Parbhani district of Maharastra (India). Indian J. Med. Res., Jan; 93:6-11.
- Mondal, R., Pemola Devi, N. and Jauhari, R.K. (2014). Occurrence of Aedes mosquitoes (Diptera: Culicidae) in urban areas of Doon Valley,(Uttarakhand), India. Modern Parasitology, Edited by Neelima Gupta and D.K. Gupta, ;255-262.
- Mudin, R.N. (2015). Dengue incidence and the prevention and control program in Malaysia. The international Medical Journal of Malaysia.14(1);5-10.
- Mukhopadhayay, A.K., Patnaik.,S.K.and Babu, P.S.(2006). Susceptibility status of some culicine mosquito to insects in Rajamundry town of A.P. Ind. J.Vector Born Dis., 43; 39-41.
- Murrell, E.G., Juliano, S.A. (2008). Detritus type alters the outcome of interspeciific competition between Aedes aegypti and Aedes albopictus (Diptera; Culicidae). J. Med.Entomol; 45; 375-83.
- Nagpal, B.N., Gupta, S.K., Ahamim, A., Kumar, V.,Anushrita, Singh, H., Saxena, R., Singh, V.P.,Srivastava, A., Bisht, B., Tuli, N.R., Singh, R.N. and Valecha, N.(2016). Identification of key containers of Aedes breeding- a cornerstone to control strategies of dengue in Delhi, India. Dengue Bull, 39,87-99.
- Prophiro, J.S., Silva, O.S., Luna, J.E., Piccoli, C.F., Kanis, L.A. and Silva, M.A.(2011).Aedes aegypti and Ae. albopictus(Diptera: Culicidae) coexistence and susceptibility to temphos in municipalities with occurrence of dengue and differentiated characteristics of urbanization. Rev. Soc. Bras.Med.Trop., 44(3); 300-5.
- Rajendran, G., Amalraj, D., Das, L.K., Ravi, R. and Das, P.K. (2006). Epidemiological and entomological investigation of Dengue fever in Sulurpet, Andhra Pradeh, India. Dengue Bull., 30; 93-98.
- Ramakrishnan, S.P., Geljand, H.M., Bose, P.N., Sehgal, P.N. and Mukherjee, R.N.(1964). The epidemic of acute haemorrhagic fever in Calcutta, in 1963, Epidemiological inquiry. Ind. J. Med. Res., 52 : 633- 650.
- Randolph, S.E. and Rogers,D.J. (2010). The arrival, establishment and spread of exotic diseases; patterns and predictions. Nat. Rev.Microbiol, 8: 361-371.
- Selvi, S., Edah, M.A., Nazni, W.A., Lee, H.L.,Tyagi, B.K.,Safian, A.M. and Azhari, A.H.(2010). Insecticide susceptibility and resistance development in malathion selected Aedes albopictus (skuse). Tropical Biomedicine 27(3): 534-550.
- Shaikh, F.S., Karikatti, S., Patil, M.S. and Gungi,P.(2017): An entomological survey as a tool for control of dengue vector during an outbreak situation. Annals of Community Health,5 (1): 14-18.
- Sharma, K., Angel, B., Singh, H., Purohit, A. and Joshi, V. (2008). Entomological studies for surveillance and prevention of dengue in arid and semiarid districts of Rajasthan. J. Vector Borne Dis., 45(2); 124-32.
- Ukpai, O.M. and Ekedo, C.M. (2018). Insecticide susceptibility status of Aedes aegypti in Umudike, IkwuanoLgaAbia state, Nigeria. Anim. Res. Int. 15(3); 3082-3089.
- Vijayakumar, K., Kumar,T.K.S., Nujum, Z.T., Umarul, F. and Kuriakose, A.(2014). A study on container breeding mosquitoes with special references to Aedes (Stegomyia) aegypti and Aedes albopictus in Thiruvanthapuram district, India.J.Vector Borne Dis., 51, March; 27-32.
- Vikram, K. Nagpal, B.N. Pande, V., Srivastava, A., Saxena, R. et al., (2016). An epidemiological study of dengue in Delhi, India. Acta Tropica, 153, 21-27.