A Study on Numerous Techniques of Chromatographic Evaluation

Exploring the Methods and Principles of Chromatographic Evaluation

Authors

  • Mukesh Kumar Gupta Research Scholar Author
  • Dr. Pushpendra Sharma Head of Department Author

Keywords:

chromatographic evaluation techniques, partitioning, sample, stationary phase, mobile phase, liquid chromatography, column, pump, detector, molecules, structures, size, charged groups, polar groups, non-polar groups, affinity column, chromatographic peaks

Abstract

Chromatographic Evaluation Techniques utilize partitioning a sample between two phases, one of which is the stationary phase and the other one is the mobile phase. Equipment for liquid chromatography includes a column, where separation takes places, pump, which generates a mobile phase flow, and detector, where the separated compounds are detected. Chromatography is a method which separates molecules based on differences in their structures, namely in size, in presence of charged, polar, and non-polar groups or moieties interacting specifically with an affinity column. After separation in a chromatographic column, particles are eluted to the detector and they are detected as chromatographic peaks.

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Published

2016-11-01

Issue

Vol. 12 No. 24 (2016): Journal of Advances in Science and Technology (JAST)

Section

Articles