Study on Characterization of Plasmids from Bacteria that use Polycyclic Aromatic Hydrocarbons: Efficient Bioremediation of Polycyclic Aromatic Hydrocarbons using Bacterial Consortia and Plasmids

Abstract

Polycyclic aromatic hydrocarbons (PAH) are carcinogenic to humans, animals, and plants.Polycyclic aromatic hydrocarbon concentrations have been steadily growing in the environment, includingwater, soil, air, sediments, marine water, and vegetables. A sustainable method will be the formation ofconsortia of efficient polycyclic aromatic hydrocarbons degraders for the total removal of polycyclicaromatic hydrocarbons from polluted locations. The hydrocarbon polluted site soil sample and subsurfacesoil sample were utilised for bacterial species separation by enrichment.The 2,6-Dichlorophenolindophenol (2,6-DCPIP) test was used to screen for efficient polycyclic aromatic hydrocarbon degraders,and potential polycyclic aromatic hydrocarbon degraders were found using 16S-rDNA sequencing. Thedetected polycyclic aromatic hydrocarbons degrader was chosen for bioremediation research based onthe catabolic route employed for catabolism of high molecular weight-polycyclic aromatic hydrocarbonssuch as pyrene and chrysene, as well as the examination of catabolic plasmid properties. On the basis ofhighest hydrocarbon degradability, consortia of high molecular weight-polycyclic aromatichydrocarbons degrading bacteria were formed.The effective polycyclic aromatic hydrocarbon degraderswere found to be Paracoccus denitrificans C5, Bacillus cereus C7, Rhodococcus pyridinivorans A1 andPseudomonas stutzeri G11. Among the various bacterial consortia developed, the consortia generatedfrom Paracoccus denitrificans C5 and Rhodococcus pyridinivorans C7 show the most promise. Hence itis concluded that the consortia formed by efficient polycyclic aromatic hydrocarbons digesting bacteriawith new plasmids is effective for polycyclic aromatic hydrocarbon bioremediation.