Identification and Characterization the Virus Isolate Prevalent In Pea Growing Localities by DAC ELISA Technique: Investigations on the identification and characterization of a prevalent virus isolate causing mosaic disease in peas

Abstract

The present investigations were undertaken to identify virus responsible for causing mosaic disease in pea (Pisum satium L.) under natural condition in Shahjahanpur. An attempt was also made to find out the resistant sources through field screening of available germplasm. The virus isolate was transmissible through sap, seed and aphid vectors Acyrthosiphon pisum and Myzus persicae. Seed transmission of virus isolate checked by grow out test and DAC ELISA technique revealed that virus is seed transmissible with a transmission rate of 20 percent. It was detected in embryo not in seed coat of seeds harvested from infected plants (var. PB-89). Thus, the virus under report is transmissible through sap, seed and aphid vectors.