An Analysis upon Isolation and Antibacterial Activities of Actinomycetes from Air: Isolation and Antibacterial Activities of Actinomycetes from Air: Analysis and Evaluation

Abstract

The main focus of this study was to isolate some antibiotic producing actinomycetes strains from air. The air sample used was dark brown and sandy, with no vegetation covering (bare). Isolation of air actinomycetes was done by culture-dependent methods and isolates were tested for antibiotic production on selected indicator bacteria plates. The results indicated that a total of 3.56 × 105 actinomycetes colonies were isolated per gram of dry air. Furthermore, microscopic examination of the isolates indicated 6 major colony types (CTs) in the air. Only 3 CTs were found to be active against one or more indicator bacteria, with inhibition zones that ranged from 7 mm to 12.5 mm in diameter. From the results, it was suggested that the low yield of antibiotic producing actinomycetes isolates obtained in this study, could be improved by employing a combination of several molecular analysis methods.Actinomycetes are one of the most attractive sources of antibiotics. In the present studies, total of 15 strains were isolated from Dr. Ram Manohar Lohia Hospital and RML Park in Lucknow, U.P India. Isolated strains were identified for their antibacterial activity but only six isolate showed good result, they were evaluated for their inhibitory activity on 3 strains of microorganism (E. coli, P. aeruginosa and S. aureus). Isolation of Actinomycetes strain was obtained by serial dilution method and grown on actinomycetes isolation agar. Antibacterial compounds were produced by submerged fermentation and activity of compounds were checked against bacterial culture by antibiogram analysis where intracellular and extracellular compounds showed positive result, compare to intracellular compounds, extracellular compounds was showing best result which was 30 mm zone of inhibition against S. aureus and MIC was found to be 0.0009 mg/ml.